01945nam a2200265Ia 4500 MX-MdCICY 20260521091348.0 CICY B-13407 A novel staining method for detecting phytase activity Journal of MicroBiological Methods, 39(1), p.17-22, 1999 Differential agar media for the detection of microbial phytase activity use the disappearance of precipitated calcium or sodium phytate as an indication of enzyme activity. When this technique was applied to the study of ruminal bacteria, it became apparent that the method was unable to differentiate between phytase activity and acid production. Strong positive reactions (zones of clearing around microbial colonies)observed for acid producing, anaerobic bacteria, such as Streptococcus bovis, were not corroborated by subsequent quantitative assays. Experimentation revealed that acidic solutions generated false positive results on the selected differential medium. Empirical studies undertaken to find a solution to this limitation determined the false positive results could be eliminated through a two step counterstaining treatment (cobalt chloride and ammonium molybdate/ammonium vanadate)which reprecipitates acid solubilized phytate. This report discusses the application of the developed two step counterstaining treatment for the screening of phytase producing ruminal bacteria as well as its use in phytase zymogram assays. PHYTASE PHYTIC ACID PLATE SCREENING STAINING ZYMOGRAM Bae, H.D. Yanke, L.J. Cheng, K.-J. Selinger, L.B. https://drive.google.com/file/d/1PeNLxuVLQeNv4jxcaOiHGI8BInnYbzcr/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 23546 23546 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-13407 2025-06-25 15:39:22 2025-06-25 REF1