Ruiz-Herrera, J. Ruiz-Medrano, R. Dominguez, A. text xx 9999 monographic und

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We have advanced the hypothesis that polyamines affect DNA methylation and thus promote the expression of developmentally controlled genes. We demonstrate that the activity of cytosine-DNA methyltransferases HpaII, HhaI, HaeIII and SssI is inhibited by physiological concentrations of polyamines. On the other hand, activity of the adenine-DNA methyltransferase EcoRI, and restriction enzymes HpaII, HhaI, HaeIII and EcoRI, is insensitive to polyamine concentrations up to 40 mM. Our results indicate that the effect of polyamines on cytosine- DNA methyltranferases is rather selective and suggest a possible mode of action in vivo. PUTRESCINE SPERMIDINE DNA-METHYLATION RESTRICTION ENZYME https://drive.google.com/file/d/1Nw1MdMkeeIWcT168g2nIYrsWoEVuCZ9K/view?usp=drivesdk https://drive.google.com/file/d/1Nw1MdMkeeIWcT168g2nIYrsWoEVuCZ9K/view?usp=drivesdk 250602 20260521091326.0