01317nam a2200205Ia 4500003001000000005001700010040000900027245006600036490004200102520063700144650001500781650001500796650002000811650002300831700002100854700002100875700001800896856015600914008004101070MX-MdCICY20260521091326.0  cCICY10aSelective inhibition of cytosine-DNA methylases by polyamines0 vFEBS Letters, 357(2), p.192-196, 19953 aWe have advanced the hypothesis that polyamines affect DNA methylation and thus promote the expression of developmentally controlled genes. We demonstrate that the activity of cytosine-DNA methyltransferases HpaII, HhaI, HaeIII and SssI is inhibited by physiological concentrations of polyamines. On the other hand, activity of the adenine-DNA methyltransferase EcoRI, and restriction enzymes HpaII, HhaI, HaeIII and EcoRI, is insensitive to polyamine concentrations up to 40 mM. Our results indicate that the effect of polyamines on cytosine- DNA methyltranferases is rather selective and suggest a possible mode of action in vivo.14aPUTRESCINE14aSPERMIDINE14aDNA-METHYLATION14aRESTRICTION ENZYME12aRuiz-Herrera, J.12aRuiz-Medrano, R.12aDominguez, A.40uhttps://drive.google.com/file/d/1Nw1MdMkeeIWcT168g2nIYrsWoEVuCZ9K/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d