Costas, C. Sanchez, M. Stroud, H. Yu, Y. Oliveros, J.C. Feng, S. Benguria, A. Benguria, A. Zhang, X. Solano, R. Jacobsen, S.E. Gutierrez, C. text xx 9999 monographic und

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Genome integrity requires faithful chromosome duplication. Origins of replication, the genomic sites at which DNA replication initiates, are scattered throughout the genome. Their mapping at a genomic scale in multicellular organisms has been challenging. In this study we profiled origins in Arabidopsis thaliana by high-throughput sequencing of newly synthesized DNA and identified ~1,500 putative origins genome-wide. This was supported by chromatin immunoprecipitation and microarray (ChIP-chip)experiments to identify ORC1- and CDC6-binding sites. We validated origin activity independently by measuring the abundance of nascent DNA strands. The midpoints of most A. thaliana origin regions are preferentially located within the 5_O half of genes, enriched in G+C, histone H2A.Z, H3K4me2, H3K4me3 and H4K5ac, and depleted in H3K4me1 1 and H3K9me2. Our data help clarify the epigenetic specification of DNA replication origins in A. thaliana and have implications for other eukaryotes. https://drive.google.com/file/d/1J7YDYNbMLA6RRqUOEeyZkSlkpqYQXSAU/view?usp=drivesdk https://drive.google.com/file/d/1J7YDYNbMLA6RRqUOEeyZkSlkpqYQXSAU/view?usp=drivesdk 250602 20260521091325.0