01937nam a2200301Ia 4500 MX-MdCICY 20260521091325.0 CICY B-12682 Genome-wide mapping of Arabidopsis thaliana origins of DNA replication and their associated epigenetic marks Nature Structural & Molecular Biology, 18, p.395-400, 2011 Genome integrity requires faithful chromosome duplication. Origins of replication, the genomic sites at which DNA replication initiates, are scattered throughout the genome. Their mapping at a genomic scale in multicellular organisms has been challenging. In this study we profiled origins in Arabidopsis thaliana by high-throughput sequencing of newly synthesized DNA and identified ~1,500 putative origins genome-wide. This was supported by chromatin immunoprecipitation and microarray (ChIP-chip)experiments to identify ORC1- and CDC6-binding sites. We validated origin activity independently by measuring the abundance of nascent DNA strands. The midpoints of most A. thaliana origin regions are preferentially located within the 5_O half of genes, enriched in G+C, histone H2A.Z, H3K4me2, H3K4me3 and H4K5ac, and depleted in H3K4me1 1 and H3K9me2. Our data help clarify the epigenetic specification of DNA replication origins in A. thaliana and have implications for other eukaryotes. Costas, C. Sanchez, M. Stroud, H. Yu, Y. Oliveros, J.C. Feng, S. Benguria, A. Benguria, A. Zhang, X. Solano, R. Jacobsen, S.E. Gutierrez, C. https://drive.google.com/file/d/1J7YDYNbMLA6RRqUOEeyZkSlkpqYQXSAU/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 22828 22828 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-12682 2025-06-25 15:39:05 2025-06-25 REF1