02349nam a2200325Ia 4500003001000000005001700010040000900027090001200036245011700048490006000165520123700225650001401462650001901476650002601495650002301521700001301544700001601557700001601573700001501589700001801604700001801622700001701640700001701657700001801674856015601692942001401848008004101862999001701903952010301920MX-MdCICY20260521091323.0  cCICY  aB-1263010aIdentification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity0 vInternational ImmunoPharmacology, 5(2), p.271-279, 20053 aPolysaccharides isolated from the gel of Aloe species have been known to have diverse biological activities, including immunomodulatory and antitumor activities. The molecular size-immunomodulatory activity relationship of modified Aloe polysaccharide (MAP)was examined in this study. Crude MAP (G2E1)was prepared from the gel of Aloe vera that was partially digested with cellulase. Proteins in crude MAP were removed by passage through a DEAE-Sephacel column, and then the protein-free MAP (G2E1D)was further separated into three fractions, G2E1DS3 molecular weight (MWz400 KDa), G2E1DS2 (5 KDaVMWV400 KDa), G2E1DS1 (MWV5 KDa), by Sephacryl column chromatography and ultrafiltration. Immunomodulatory activities of MAP preparations were examined on a mouse macrophage cell line, RAW 264.7 cells, and in ICR strain of mouse implanted with sarcoma 180 cells. We found that polysaccharides between 400 and 5 KDa exhibit the most potent macrophage-activating activity as determined by increased cytokine production, nitric oxide release, expression of surface molecules, and phagocytic activity. In accordance with the in vitro activity, polysaccharides between 400 and 5 KDa also exhibited the most potent antitumor activity in vivo.14aALOE VERA14aPOLYSACCHARIDE14aMACROPHAGE ACTIVATION14aANTITUMOR ACTIVITY12aSun-A Im12aSun-Tack Oh12aSukgil Song12aMi-Ran Kim12aDong-Seon Kim12aSung-Sick Woo12aTae Hyung Jo12aTae Hyung Jo12aChong-Kil Lee40uhttps://drive.google.com/file/d/1BN4CcC6a5nmxM95hZAq-jS3Xyd6iEDJ4/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx  2LoccREF1250602s9999    xx |||||s2   |||| ||und|d  c22776d22776  00102Loc40708F1aCICYbCICYcREd2025-06-25l0oB-12630r2025-06-25 15:39:02w2025-06-25yREF1