01989nam a2200277Ia 4500 MX-MdCICY 20260521091319.0 CICY B-12499 Genetically pyramiding protease-inhibitor genes for dual road-spectrum resistance against insect and phytopathogens in transgenic tobacco Plant Biotechnology Journal, 8(1), p.65-75, 2010 Protease inhibitors provide a promising means of engineering plant resistance against attack by insects and pathogens. Sporamin (trypsin inhibitor)from sweet potato and CeCPI (phytocystatin)from taro were stacked in a binary vector, using pMSPOA (a modified sporamin promoter)to drive both genes. Transgenic tobacco lines of T0 and T1 generation with varied inhibitory activity against trypsin and papain showed resistance to both insects and phytopathogens. Larvae of Helicoverpa armigera that ingested tobacco leaves either died or showed delayed growth and development relative to control larvae. Transgenic tobacco-overexpressing the stacked genes also exhibited strong resistance against bacterial soft rot disease caused by Erwinia carotovora and damping-off disease caused by Pythium aphanidermatum. Thus, stacking protease-inhibitor genes, driven by the wound and pathogen responsive pMSPOA promoter, is an effective strategy for engineering crops to resistance against insects and phytopathogens. SPORAMIN CECPI (TARO CYSTATIN) SYNTHETIC WOUND-INDUCIBLE&PROMOTER (PMSPOA) TRANSGENIC TOBACCO INSECT RESISTANCE ANTIBACTERIAL ACTIVITY ANTIFUNGAL ACTIVITY Senthilkumar, R. Cheng, C. Yeh, K. https://drive.google.com/file/d/1Ela5eGXdmJXX1d2Y7CVTl2K2Nvu6oXT6/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 22645 22645 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-12499 2025-06-25 15:38:55 2025-06-25 REF1