01765nam a2200217Ia 4500003001000000005001700010040000900027245006900036490003600105520104400141650002901185650003101214650004201245700001201287700001301299700001301312700001301325700001201338856015601350008004101506MX-MdCICY20260521091311.0  cCICY10aConstruction of two Gateway vectors for gene expression in fungi0 vPlasmid, 62(2), p.128-133, 20093 aWe report the construction of two Gateway fungal expression vectors pCBGW and pGWBF. The pCBGW was generated by introducing an expression cassette, which consists of a Gateway recombinant cassette (attR1-Cmr-ccdB-attR2)under the control of fungal promoter PgpdA and a terminator TtrpC, into the multiple cloning site of fungal vector pCB1004. The pGWBF is a binary vector, which was generated from the plant expression vector pGWB2 by replacing the CaMV35S promoter with PgpdA. The pGWBF can be transformed into fungi efficiently with Agrobacterium-mediated transformation. The applicability of two newly constructed vectors was tested by generating the destination vectors pGWBF-GFP and pCBGW-GFP and examining the expression of GFP gene in Trichoderma viride and Gibberella fujikuroi, respectively. Combining with the advantage of Gateway cloning technology, pCBGW and pGWBF will be useful in fungi for large-scale investigation of gene functions by constructing the interested gene destination/expression vectors in a highthroughput way14aFUNGAL EXPRESSION VECTOR14aGATEWAY CLONING TECHNOLOGY14aAGROBACTERIUM-MEDIATED TRANSFORMATION12aZhu, T.12aWang, W.12aYang, X.12aWang, K.12aCui, Z.40uhttps://drive.google.com/file/d/1hKtyL-T2fdN5WJbWYfb1OnJCtvmg1PuS/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d