02542nam a2200337Ia 4500 MX-MdCICY 20260521091305.0 CICY B-12089 Peripheral nerve regeneration with sustained release of poly(phosphoester)microencapsulated nerve growth factor within nerve guide conduits BioMaterials, 24(13), p.2405-2412, 2003 Prolonged delivery of neurotrophic proteins to the target tissue is valuable in the treatment of various disorders of the nervous system. We have tested in this study whether sustained release of nerve growth factor (NGF)within nerve guide conduits (NGCs), a device used to repair injured nerves, would augment peripheral nerve regeneration. NGF-containing polymeric microspheres fabricated from a biodegradable poly(phosphoester)(PPE)polymer were loaded into silicone or PPE conduits to provide for prolonged, site-specific delivery of NGF. The conduits were used to bridge a 10mm gap in a rat sciatic nerve model. Three months after implantation, morphological analysis revealed higher values of fiber diameter, fiber population and fiber density and lower Gratio at the distal end of regenerated nerve cables collected from NGF microsphere-loaded silicone conduits, as compared with those from control conduits loaded with either saline alone, BSA microspheres, or NGF protein without microencapsulation. Beneficial effects on fiber diameter, G-ratio and fiber density were also observed in the permeable PPE NGCs. Thus, the results confirm a longterm promoting effect of exogenous NGF on morphological regeneration of peripheral nerves. The tissue-engineering approach reported in this study of incorporation of a microsphere protein release system into NGCs holds potential for improved functional recovery in patients whose injured nerves are reconstructed by entubulation. NERVE GROWTH FACTOR POLYMERIC MICROSPHERE NERVE GUIDE CONDUITS POLY(PHOSPHOESTER) NERVE REGENERATION Xu, X. Yee, W. Hwang, P.Y.K. Yu, H. Wan, A.C.A. Gao, S. Boon, K. Boon, K. Leong, K.W. Wang, W. https://drive.google.com/file/d/1wPII1_MO5ZClJbEpfPVHuww_Kubaa1A3/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 22239 22239 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-12089 2025-06-25 14:07:03 2025-06-25 REF1