02104nam a2200241Ia 4500 MX-MdCICY 20260521091245.0 CICY B-11434 Effects of Culture Conditions on Cell Division and Composition of Regenerated Cell Walls in Vinca rosea Protoplasts Plant & Cell Physiology, 23, p.249-255, 1982 Protoplasts prepared from suspension-cultured Vinca rosea cells were grown in a liquid medium, and the effects of osmolarity and growth regulators on cell division and on the composition of regenerated cell walls were investigated. The concentration of mannitol optimal for cell division was 0.3-0.4 M. The presence of 2,4-D was essential for cell division, and 6-benzylaminopurine (BAP)enhanced cell division at concentrations of 0.03-0.1 ppm. However, the composition of regenerated cell walls was abnormal under suspension culture; the predominant sugar was glucose, indicating that the regenerated cell walls consisted mostly of glucans, and that the other cell-wall components were released into the medium. Mannitol, 2,4-D, and BAP at various concentrations did not significantly affect the sugar composition of the regenerated cell walls. Compared with liquid culture, cell division was stimulated when protoplasts were cultured on agar, and their regenerated cell walls had a composition similar to that of the original culture. The importance of the physical environment for the deposition of polysaccharide components in cell walls and the interrelationship between cell division and the regeneration of cell walls by protoplasts are discussed. CELL DIVISION CELL-WALL REGENERATION POLYSACCHARIDES (CELL WALL) PROTOPLAST CULTURE (VINCA) VINCA ROSEA Takeuchi, Y. Komamine, A. https://drive.google.com/file/d/1UbhcsmFUmF5cBiAVXklDPq6E7InOJ_-w/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 21592 21592 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-11434 2025-06-25 14:06:50 2025-06-25 REF1