Catalytic Electron Transport in Chromatium Vinosum [NiFe]-Hydrogenase: Application of Voltammetry in Detecting Redox-Active Centers and Establishing That Hydrogen Oxidation Is Very Fast Even at Potentials Close to the Reversible H+/H2 Value 

 -  BioChemistry, 38, p.8992-8999, 1999 .
<br/><br/> The nickel-iron hydrogenase from Chromatium Vinosum adsorbs at a pyrolytic graphite edgeplane (PGE)electrode and catalyzes rapid interconversion of H+ (aq)and H2 at potentials expected for the half-cell reaction 2H+ a H2, i.e., without the need for overpotentials. The voltammetry mirrors characteristics determined by conventional methods, while affording the capabilities for exquisite control and measurement of potential-dependent activities and substrate-product mass transport. Oxidation of H2 is extremely rapid; at 10