Sudheer Pamidimarri, D. V. N. Singh, S. Mastan, S.G. Patel, J. Reddy, M.P. text xx 9999 monographic und

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Jatropha curcas L., a multipurpose shrub has acquired significant economic importance for its seed oil which can be converted to biodiesel, is emerging as an alternative to petro-diesel. The deoiled seed cake remains after oil extraction is toxic and cannot be used as a feed despite having best nutritional contents. No quantitative and qualitative differences were observed between toxic and non-toxic varieties of J. curcas except for phorbol esters content. Development of molecular marker will enable to differentiate non-toxic from toxic variety in a mixed population and also help in improvement of the species through marker assisted breeding programs. The present investigation was undertaken to characterize the toxic and non-toxic varieties at molecular level and to develop PCR based molecular markers for distinguishing non-toxic from toxic or vice versa. The polymorphic markers were successfully identified specific to non-toxic and toxic variety using RAPD and AFLP echniques. Totally 371 RAPD, 1,442 AFLP markers were analyzed and 56 (15.09 JATROPHA CURCAS L NON-TOXIC TOXIC RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) AFLP (AMPLIFIED FRAGMENT LENGTH POLYMORPHISM) MICROSATELLITES https://drive.google.com/file/d/1HZkYGRw7HEuyu9zyUssMlldaOYrVNI_x/view?usp=drivesdk https://drive.google.com/file/d/1HZkYGRw7HEuyu9zyUssMlldaOYrVNI_x/view?usp=drivesdk 250602 20260521091225.0