02077nam a2200289Ia 4500 MX-MdCICY 20260521091225.0 CICY B-10833 Molecular characterization and identification of markers for toxic and non-toxic varieties of Jatropha curcas L. using RAPD, AFLP and SSR markers Mol Biol Rep, 36(6), p.1357-1364, 2009 Jatropha curcas L., a multipurpose shrub has acquired significant economic importance for its seed oil which can be converted to biodiesel, is emerging as an alternative to petro-diesel. The deoiled seed cake remains after oil extraction is toxic and cannot be used as a feed despite having best nutritional contents. No quantitative and qualitative differences were observed between toxic and non-toxic varieties of J. curcas except for phorbol esters content. Development of molecular marker will enable to differentiate non-toxic from toxic variety in a mixed population and also help in improvement of the species through marker assisted breeding programs. The present investigation was undertaken to characterize the toxic and non-toxic varieties at molecular level and to develop PCR based molecular markers for distinguishing non-toxic from toxic or vice versa. The polymorphic markers were successfully identified specific to non-toxic and toxic variety using RAPD and AFLP echniques. Totally 371 RAPD, 1,442 AFLP markers were analyzed and 56 (15.09 JATROPHA CURCAS L. NON-TOXIC TOXIC RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) AFLP (AMPLIFIED FRAGMENT LENGTH POLYMORPHISM) MICROSATELLITES Sudheer Pamidimarri, D. V. N. Singh, S. Mastan, S.G. Patel, J. Reddy, M.P. https://drive.google.com/file/d/1HZkYGRw7HEuyu9zyUssMlldaOYrVNI_x/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 21003 21003 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-10833 2025-06-25 14:06:39 2025-06-25 REF1