01779nam a2200265Ia 4500003001000000005001700010040000900027245015400036490005100190520083600241650001701077650002301094650001701117650001401134650002501148650004101173700002301214700001401237700001501251700001701266700001701283700001601300856015601316008004101472MX-MdCICY20260521091223.0  cCICY10aTetrahydrocannabinolic Acid Synthase, the Enzyme Controlling Marijuana Psychoactivity, is Secreted into the Storage Cavity of the Glandular Trichomes0 vPlant & Cell Physiology, 46, p.1578-1582, 20053 aTetrahydrocannabinolic acid (THCA)synthase is the enzyme responsible for the production of tetrahydrocannabinol (THC), the psychoactive component of marijuana (Cannabis sativa L.). We suggest herein that THCA is biosynthesized in the storage cavity of the glandular trichomes based on the following observations. (i)The exclusive expression of THCA synthase was confirmed in the secretory cells of glandular trichomes by reverse transcription- PCR (RT-PCR)analysis. (ii)THCA synthase activity was detected in the storage cavity content. (iii)Transgenic tobacco expressing THCA synthase fused to green fluorescent protein showed fluorescence in the trichome head corresponding to the storage cavity. These results also showed that secretory cells of the glandular trichomes secrete not only metabolites but also biosynthetic enzyme.14aBIOSYNTHESIS14aGLANDULAR TRICHOME14aLOCALIZATION14aMARIJUANA14aTETRAHYDROCANNABINOL14aTETRAHYDROCANNABINOLIC ACID SYNTHASE12aSirikantaramas, S.12aTaura, F.12aTanaka, Y.12aIshikawa, Y.12aMorimoto, S.12aShoyama, Y.40uhttps://drive.google.com/file/d/1qaDVBNTgSMHecsdDowVcWvLlFi0b2nRB/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d