02136nam a2200265Ia 4500 MX-MdCICY 20260521091217.0 CICY B-10566 Directing Vanillin Production From Ferulic Acid by Increased Acetyl-CoA Consumption in Recombinant Escherichia coli Biotechnology and BioEngineering, 102(1), p.200-208, 2009 The amplification of gltA gene encoding citrate synthase of TCA cycle was required for the efficient conversion of acetyl-CoA, generated during vanillin production from ferulic acid, to CoA, which is essential for vanillin production. Vanillin of 1.98 g/L was produced from the E. coli DH5a (pTAHEF-gltA)with gltA amplification in 48 h of culture at 3.0 g/L of ferulic acid, which was about twofold higher than the vanillin production of 0.91 g/L obtained by the E. coli DH5a (pTAHEF)without gltA amplification. The icdA gene encoding isocitrate dehydrogenase of TCA cycle was deleted to make the vanillin producing E. coli utilize glyoxylate bypass which enables more efficient conversion of acetyl-CoA to CoA in comparison with TCA cycle. The production of vanillin by the icdA null mutant of E. coli BW25113 harboring pTAHEF was enhanced by 2.6 times. The gltA amplification of the glyoxylate bypass in the icdA null mutant remarkably increased the production rate of vanillin with a little increase in the amount of vanillin production. The real synergistic effect of gltA amplification and icdA deletion was observed with use of XAD-2 resin reducing the toxicity of vanillin produced during culture. Vanillin of 5.14 g/L was produced in 24 h of the culture with molar conversion yield of 86.6 Lee, E. Yoon, S. Das, A. Lee, S. Li, C. Kim, J. Choi, M. Choi, M. Kim, S. https://drive.google.com/file/d/1KFa4zEOMd6OPIsW5sh6phEsd_re19OG6/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 20737 20737 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-10566 2025-06-25 14:06:34 2025-06-25 REF1