02249nam a2200301Ia 4500 MX-MdCICY 20260521091214.0 CICY B-10496 Specific functions of individual class III peroxidase genes Journal of Experimental Botany, 60, p.391-408, 2009 In higher plants, class III peroxidases exist as large multigene families (e.g. 73 genes in Arabidopsis thaliana). The diversity of processes catalysed by peroxidases as well as the large number of their genes suggests the possibility of a functional specialization of each isoform. In addition, the fact that peroxidase promoter sequences are very divergent and that protein sequences contain both highly conserved domains and variable regions supports this hypothesis. However, two difficulties are associated with the study of the function of specific peroxidase genes: (i)the modification of the expression of a single peroxidase gene often results in no visible mutant phenotype, because it is compensated by redundant genes; and (ii)peroxidases show low substrate specificity in vitro resulting in an unreliable indication of peroxidase specific activity unless complementary data are available. The generalization of molecular biology approaches such as whole transcriptome analysis and recombinant DNA combined with biochemical approaches provide unprecedented tools for overcoming these difficulties. This review highlights progress made with these new techniques for identifying the specific function of individual class III peroxidase genes taking as an example the model plant A. thaliana, as well as discussing some other plants. AUXIN CATABOLISM DEFENCES GENES ISOFORMS LIGNIFICATION PROTEOMIC REGULATION PATHWAYS STRESSES TRANSCRIPTOME ANALYSIS TRANSGENIC PLANTS Cosio, C. Dunand, C. https://drive.google.com/file/d/1FTtITDddIYjxIucdHxw2b0RlAwBZ3Ita/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 20667 20667 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-10496 2025-06-25 14:06:32 2025-06-25 REF1