01762nam a2200241Ia 4500003001000000005001700010040000900027245009200036490002900128520099400157650001801151650002101169650001401190650001801204650001801222650001801240700001701258700001801275700001501293700001501308856015601323008004101479MX-MdCICY20260521091211.0  cCICY10aDNA transfer from chloroplast to nucleus is much rarer in Chlamydomonas than in tobacco0 vGene, 316, p.33-39, 20033 aBy transforming chloroplasts with an antibiotic-resistance gene under the control of a nuclear-specific promoter, we employed a selection scheme to detect the transfer of DNA from the chloroplast to the nucleus in the green alga Chlamydomonas reinhardtii. Among several billion homoplasmic cells tested, we were unable to detect any stable nuclear integration of chloroplast DNA under normal growth conditions or under stress conditions. This contrasts with results reported for the transfer of DNA from chloroplast to nucleus in higher plants and from mitochondrion to nucleus in Saccharomyces cerevisiae. Furthermore, we were unable to detect chloroplast DNA-derived sequences among nuclear genome data for C. reinhardtii, which also contrasts with the situation in higher plants. Taken together, these findings suggest that there is presently little, if any, movement of DNA from chloroplast to nucleus in C. reinhardtii, which may reflect the ultrastructure of the C. reinhardtii cell.14aENDOSYMBIOSIS14aGENOME EVOLUTION14aORGANELLE14aGENE TRANSFER14aTRANSPOSITION14aTRANSLOCATION12aLister, D.L.12aBateman, J.M.12aPurton, S.12aHowe, C.J.40uhttps://drive.google.com/file/d/17QANVhNrJk65K6BFllCgO1olff4aeaxK/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d