02396nam a2200265Ia 4500003001000000005001700010040000900027090001100036245010200047490008300149520140300232650002701635650002101662650002701683650002501710700001301735700001801748700001801766700001601784856015601800942001401956008004101970999001702011952010202028MX-MdCICY20260521091158.0  cCICY  aB-996310aPurification and Characterization of Three Antifungal Proteins from Cheeseweed (Malva parviflora)0 vBioChemical and Biophysical Research Communications, 282(5), p.1224-1228, 20013 aThree potent antimicrobial proteins were purified from cheeseweed (Malva parviflora)seeds. These antimicrobial proteins, named CW-3, CW-4, and CW-5, showed different antimicrobial spectrum and potency compared to the two heterologous antimicrobial proteins (CW-1 and CW-2)purified previously. CW-3 and CW-4 possess antimicrobial activities against Phytophthora infestans (Pi), but not Fusarium graminearum (Fg). A database search indicated that CW-3 shares high homology to cotton vicilin, an abundant seed storage protein. CW-4 shares homology to 2S albumin, another seed storage protein from cotton. CW-5 has antimicrobial activity against Fg, but no activity against Pi was observed at protein concentration up to 50 ppm. Under low salt condition, CW-5 showed potent antimicrobial activity against Fg, but under high salt condition, the antimicrobial activity was drastically diminished. Database search indicated that CW-5 has high homology to a lipid transfer protein from grape. The IC50 values of the three purified antimicrobial proteins under both low and high salt conditions were determined. The isolation of five antimicrobial proteins for the first time from a single plant source provides further understanding of the plant innate defense system and insight on how plants evolve their complex and complementary antimicrobial system that is important in the early stage of development.14aANTIMICROBIAL PROTEINS14aMALVA PARVIFLORA14aPHYTOPHTHORA INFESTANS14aFUSARIUM GRAMINEARUM12aWang, X.12aBunkers, G.J.12aWalters, M.R.12aThoma, R.S.40uhttps://drive.google.com/file/d/1_4aeF_lIAOjbCzGRqL9e7kMJ9Z9AtNvZ/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx  2LoccREF1250602s9999    xx |||||s2   |||| ||und|d  c20144d20144  00102Loc40708F1aCICYbCICYcREd2025-06-25l0oB-9963r2025-06-25 14:06:22w2025-06-25yREF1