01707nam a2200289Ia 4500003001000000005001700010040000900027090001100036245014900047490008200196520063700278650002400915650001100939650001300950650001500963650001500978700001400993700001601007700002201023700001601045700002601061856015601087942001401243008004101257999001701298952010201315MX-MdCICY20260521091154.0  cCICY  aB-982110aDevelopment and Validation of an HPLC Method for the Characterization and Assay of the Saponins from Ilex paraguariensis A. St.-Hil (Mate)Fruits0 vJournal of Liquid Chromatography & Related Technologies, 30(1), p.87-95, 20073 aTwo improved HPLC methods for the characterization and assay of the saponin content in green fruits of Ilex paraguariensis (erva-mate)are described. Both HPLC methods consisted of isocratic separation at room temperature, using a RP-18 column with UV-Vis detection. The first system was intended for the characterization of the main I. paraguariensis saponin fraction, while the second one was specifically developed and validated for its quantitation. Matesaponin-3 was used as external standard. From the freeze dried extract, ten peaks were characterized as saponins by TLC and HPLC analysis. The matesaponin-3 content was 5.13 g14aILEX PARAGUARIENSIS14aFRUITS14aSAPONINS14aHPLC ASSAY14aVALIDATION12aPavei, C.12aGuzatto, P.12aRos Petrovick, P.12aGosmann, G.12aGonzález-Ortega, G.40uhttps://drive.google.com/file/d/1mu6BQfGbt9BZyE0CHe3LlkhQOFi5dW5S/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx  2LoccREF1250602s9999    xx |||||s2   |||| ||und|d  c20006d20006  00102Loc40708F1aCICYbCICYcREd2025-06-25l0oB-9821r2025-06-25 14:06:20w2025-06-25yREF1