Pavei, C. Guzatto, P. Ros Petrovick, P. Gosmann, G. González-Ortega, G. text xx 9999 monographic und

electronic

Two improved HPLC methods for the characterization and assay of the saponin content in green fruits of Ilex paraguariensis (erva-mate)are described. Both HPLC methods consisted of isocratic separation at room temperature, using a RP-18 column with UV-Vis detection. The first system was intended for the characterization of the main I. paraguariensis saponin fraction, while the second one was specifically developed and validated for its quantitation. Matesaponin-3 was used as external standard. From the freeze dried extract, ten peaks were characterized as saponins by TLC and HPLC analysis. The matesaponin-3 content was 5.13 g ILEX PARAGUARIENSIS FRUITS SAPONINS HPLC ASSAY VALIDATION https://drive.google.com/file/d/1mu6BQfGbt9BZyE0CHe3LlkhQOFi5dW5S/view?usp=drivesdk https://drive.google.com/file/d/1mu6BQfGbt9BZyE0CHe3LlkhQOFi5dW5S/view?usp=drivesdk 250602 20260521091154.0