Lu, Y. Luo, J. Xu, D. Huang, X. Kong, L. text xx 9999 monographic und

electronic

High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MSn)was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS1-3 in positive ion mode. The MSn spectra of the [MRH]R ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-b-D-quinovopyranoside and solagenin 6-O-[b-D-xylopyranosyl-(1!3)-O-b-D-quinovopyranoside] https://drive.google.com/file/d/1G5SOMufsx4Yz7jiHKxiZDonlT90c3TdH/view?usp=drivesdk https://drive.google.com/file/d/1G5SOMufsx4Yz7jiHKxiZDonlT90c3TdH/view?usp=drivesdk 250602 20260521091153.0