01657nam a2200217Ia 4500 MX-MdCICY 20260521091153.0 CICY B-9820 Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/ evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry Rapid Communications in Mass Spectrometry, 22(16), p.2447-2452, 2008 High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MSn)was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS1-3 in positive ion mode. The MSn spectra of the [MRH]R ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-b-D-quinovopyranoside and solagenin 6-O-[b-D-xylopyranosyl-(1!3)-O-b-D-quinovopyranoside] Lu, Y. Luo, J. Xu, D. Huang, X. Kong, L. https://drive.google.com/file/d/1G5SOMufsx4Yz7jiHKxiZDonlT90c3TdH/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 20005 20005 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-9820 2025-06-25 14:06:20 2025-06-25 REF1