02077nam a2200289Ia 4500003001000000005001700010040000900027090001100036245013200047490006900179520102600248650001601274650000901290650001401299650002801313650002401341700002001365700001401385700001801399700002101417700001901438856015601457942001401613008004101627999001701668952010201685MX-MdCICY20260521091148.0  cCICY  aB-966010aComparative analysis of [FeFe]hydrogenase from Thermotogales indicates the molecular basis of resistance to oxygen inactivation0 vInternational Journal of Hydrogen Energy, 33(2), p.570-578, 20083 aSeveral Thermotogales, previously reported as being strict anaerobes, have demonstrated the ability to grow and produce hydrogen in the presence of moderate amounts of molecular oxygen. Thermotoga neapolitana seems to be less sensitive to O2 than other members of this order, including Thermotoga maritima, whose hydrogenase has been purified and characterized. Instead, the enzyme responsible for the hydrogen production by T. neapolitana has not yet been identified. After the recent sequencing of the T. neapolitana genome, it has been possible to search for the orthologous gene responsible for this unusal hydrogenase activity. By means of in silico analysis, we built a molecular model for both T. maritima and T. neapolitana proteins and analyzed conservation, focusing on the subtle structural differences responsible for the increased oxygen resistance in the latter and underscoring two mutations (E475S and T539L)which represent a specific adaption for more effective release of hydrogen in aerobic conditions.14aBIOHYDROGEN14aHYDA14aH-CLUSTER14aHYDROPHOBIC GAS CHANNEL14aFUNCTION PREDICTION12aTosatto, S.C.E.12aToppo, S.12aCarbonera, D.12aGiacometti, G.M.12aCostantini, P.40uhttps://drive.google.com/file/d/10ipoaGpQBkKGSPJXvp822ahXrZpY4WTc/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx  2LoccREF1250602s9999    xx |||||s2   |||| ||und|d  c19846d19846  00102Loc40708F1aCICYbCICYcREd2025-06-25l0oB-9660r2025-06-25 14:06:17w2025-06-25yREF1