01963nam a2200277Ia 4500 MX-MdCICY 20260521091148.0 CICY B-9660 Comparative analysis of [FeFe]hydrogenase from Thermotogales indicates the molecular basis of resistance to oxygen inactivation International Journal of Hydrogen Energy, 33(2), p.570-578, 2008 Several Thermotogales, previously reported as being strict anaerobes, have demonstrated the ability to grow and produce hydrogen in the presence of moderate amounts of molecular oxygen. Thermotoga neapolitana seems to be less sensitive to O2 than other members of this order, including Thermotoga maritima, whose hydrogenase has been purified and characterized. Instead, the enzyme responsible for the hydrogen production by T. neapolitana has not yet been identified. After the recent sequencing of the T. neapolitana genome, it has been possible to search for the orthologous gene responsible for this unusal hydrogenase activity. By means of in silico analysis, we built a molecular model for both T. maritima and T. neapolitana proteins and analyzed conservation, focusing on the subtle structural differences responsible for the increased oxygen resistance in the latter and underscoring two mutations (E475S and T539L)which represent a specific adaption for more effective release of hydrogen in aerobic conditions. BIOHYDROGEN HYDA H-CLUSTER HYDROPHOBIC GAS CHANNEL FUNCTION PREDICTION Tosatto, S.C.E. Toppo, S. Carbonera, D. Giacometti, G.M. Costantini, P. https://drive.google.com/file/d/10ipoaGpQBkKGSPJXvp822ahXrZpY4WTc/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 19846 19846 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-9660 2025-06-25 14:06:17 2025-06-25 REF1