Sun, Y. Yang, Z. Gao, X. Li, Q. Zhang, Q. Xu, Z. text xx 9999 monographic und

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An electroporation procedure has been described for introducing plasmid DNA into Dunaliella salina cells. By this procedure, a bulk of plasmid DNA was delivered into the cells and retained for at least 3 d. Reverse transcriptase polymerase chain reaction (RT-PCR)and sequencing analyses indicated that the transcription and pre-mRNA splicing of ble gene (contributing the Zeocin resistance)were detected in the cells as early as 1 h after the electroporation. Individual colonies could retain the resistance to 10 mg/L Zeocin for at least 6 mo. Subsequent Southern blot analysis showed the existence of introduced plasmid DNA inside these colonies. However, most of the cells (approx 90 DUNALIELLA SALINA ELECTROPORATION BLE GENE ZEOCIN EPISOMAL DNA https://drive.google.com/file/d/1GZeWNNZwIMb_zvc9WZOwT2XYQEVBznbs/view?usp=drivesdk https://drive.google.com/file/d/1GZeWNNZwIMb_zvc9WZOwT2XYQEVBznbs/view?usp=drivesdk 250602 20260521091140.0