01526nam a2200289Ia 4500 MX-MdCICY 20260521091140.0 CICY B-9398 Expression of Foreign Genes in Dunaliella by Electroporation Molecular Biotechnology, 30, p.185-192, 2005 An electroporation procedure has been described for introducing plasmid DNA into Dunaliella salina cells. By this procedure, a bulk of plasmid DNA was delivered into the cells and retained for at least 3 d. Reverse transcriptase polymerase chain reaction (RT-PCR)and sequencing analyses indicated that the transcription and pre-mRNA splicing of ble gene (contributing the Zeocin resistance)were detected in the cells as early as 1 h after the electroporation. Individual colonies could retain the resistance to 10 mg/L Zeocin for at least 6 mo. Subsequent Southern blot analysis showed the existence of introduced plasmid DNA inside these colonies. However, most of the cells (approx 90 DUNALIELLA SALINA ELECTROPORATION BLE GENE ZEOCIN EPISOMAL DNA Sun, Y. Yang, Z. Gao, X. Li, Q. Zhang, Q. Xu, Z. https://drive.google.com/file/d/1GZeWNNZwIMb_zvc9WZOwT2XYQEVBznbs/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 19587 19587 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-9398 2025-06-25 14:06:12 2025-06-25 REF1