02488nam a2200349Ia 4500 MX-MdCICY 20260521091136.0 CICY B-9251 Birch PR-10c interacts with several biologically important ligands PhytoChemistry, 66(21), p.2524-2533, 2005 PR-10c is a unique member of PR-10 proteins in birch, since it is the only one known to be post-translationally modified by glutathione and is not constitutively expressed in pollen. Both reduced and S-glutathiolated forms of PR-10c show low ribonuclease activity. However, the major function of the protein is apparently not yet resolved. Our protein-ligand interaction studies with saturation transfer difference (STD)NMR revealed that PR-10c interacts with several biologically important molecules, including cytokinin, flavonoid glycosides, sterols and emodin. Competition study with deoxycholate and kinetin revealed no statistically significant binding interference, indicating that these ligands have different binding sites in PR-10c. Ligand docking studies with a molecular model of PR-10c support the STD NMR results of ligand binding and binding epitopes, suggesting that there are three potential binding sites in PR-10c: two in the hydrophobic cavity and one in the glycine-rich loop. Our docking calculations suggested that only kinetin interacts with the glycine-rich loop, the binding occurring through its adenine moiety. Clear ligand specificity could be observed in the binding of nucleotide derivatives. S-glutathiolation of PR-10c did not affect kinetin binding. The present results suggest that birch PR-10c is a multifunctional protein, which has diverse roles in plant stress responses. SATURATION TRANSFER DIFFERENCE NMR MOLECULAR MODELLING PATHOGENESIS-RELATED PROTEINS GLUTATHIOLATION CYTOKININS PHYTOSTEROLS FLAVONOIDS EMODIN Koistinen, K.M. Soininen, P. Venalainen, T.A: Hayrinen, J. Laatikainen, R. Perakyla, M. Tervahauta, A.I. Tervahauta, A.I. https://drive.google.com/file/d/123F2dVyCSMxc3Rb-Gvq5oO2MORW9yu_G/view?usp=drive_link Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 19445 19445 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-9251 2025-06-25 14:06:09 2025-06-25 REF1