02308nam a2200289Ia 4500 MX-MdCICY 20260521091132.0 CICY B-9142 Prenylation enhances cytotoxicity of apigenin and liquiritigenin in rat H4IIE hepatoma and C6 glioma cells Food and Chemical Toxicology, 45(1), p.119-124, 2006 Antioxidative as well as cytotoxic effects of the prenylated flavonoids licoflavone C (8-prenylapigenin)and isobavachin (8-prenylliquiritigenin)were investigated in comparison to the corresponding non-prenylated flavonoids (apigenin, liquiritigenin)and vitexin (apigenin-C8-glucoside)using metabolically active H4IIE hepatoma and metabolically poorly active C6 glioma cells. None of the substances showed radical scavenging activities in the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-assay nor were they effective in protection against H2O2-induced intracellular 20,70-dichlorodihydrofluorescein (H2DCF)oxidation (fluorescent probe for oxidative stress)in H4IIE and C6 cells. When the intrinsic effects of the substances were investigated, licoflavone C and isobavachin exerted a pronounced toxicity in both H4IIE (IC50 values of 42 ± 5 and 96 ± 19 lmol/L)and C6 cells (IC50 values of 37 ± 6 and 69 ± 3 lmol/L)while the non-prenylated analogues as well as the glycosylated derivate vitexin showed almost no cytotoxic effect up to 250 lmol/L. In H4IIE cells the induction of apoptotic cell death by licoflavone C and icobavachin was detected as an activation of caspase 3/7 (6- and 3.3-fold, respectively). Based on these experiments we suggest that C8-prenylation of a flavonoid enhances the cytotoxicity inducing an apoptotic cell death in H4IIE cells without affecting antioxidative properties. APOPTOSIS CYTOTOXICITY DPPH ASSAY OXIDATIVE STRESS Watjen, W. Weber, W. Lou, YJ. Wang, ZQ. Chovolou, Y. Kampkotter, A. Proksch, P. https://drive.google.com/file/d/1ClvBmkLpxqOstTKHFjYcyZNTGHSQevyq/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 19339 19339 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-9142 2025-06-25 12:47:33 2025-06-25 REF1