01770nam a2200265Ia 4500 MX-MdCICY 20260521091124.0 CICY B-8878 Emulsion PCR-based method to detect Y chromosome microdeletions Analytical BioChemistry, 367(2), p.173-178, 2007 Deletions in Y chromosome are thought to be pathologically involved in some cases of male infertility associated with azoospermia or oligozoospermia. An emulsion-based multiplex PCR method was developed for detecting Y chromosome microdeletions in infertile men and a plasma sample of pregnant women carrying a male fetus. The sensitivity of multiplex PCR in emulsion was evaluated. Conventional PCR was also carried out for comparison. A total of 13 sequence-tagged sites (STSs)distributed in the AZF region were analyzed simultaneously with this method. The SRY gene was also detected as the inner control. Results showed that Y chromosome microdeletions were found in 4 of 19 infertile patients. Also, in 1 of 63 samples collected from pregnant women, microdeletions were found in some of the detected sites. It is suggested that the emulsion PCR assay was proven to be a promising diagnostic tool and could be widely used in further clinical and academic research. EMULSIONS MULTIPLEX PCR Y CHROMOSOME MICRODELETIONS Ge, Q. Liu, Z. Bai, Y. Zhang, D. Yu, P. Lu, Z. https://drive.google.com/file/d/1lRYB7RBZhI5cKmK3S5wX4zmf1f2llgJu/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 19079 19079 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-8878 2025-06-25 12:47:28 2025-06-25 REF1