02453nam a2200301Ia 4500 MX-MdCICY 20260521091113.0 CICY B-8476 Mitochondrial proteomics reveals new insights into embryogenic competence acquisition in Carica papaya L. callus Journal of Proteomics, 252, p.104434, 2022 Understanding the mechanisms that endow a somatic cell with the ability to differentiate into a somatic embryo, which could result in numerous biotechnological applications, is still a challenge. The objective of this work was to identify some of the molecular and physiological mechanisms responsible for the acquisition of embryogenic competence during somatic embryogenesis in Carica papaya L. We performed a broad characterization of embryogenic (EC)and nonembryogenic calli (NEC)of using global and mitochondrial proteomic approaches, histomorphology, histochemistry, respiratory activity, and endogenous hormonal and hydrogen peroxide (H2O2)contents. EC and NEC presented remarkable differences in anatomical and histochemical characteristics, with EC showing a higher reactivity for the presence of proteins and neutral polysaccharides. Our results demonstrate that mitochondrial metabolism affects the embryogenic competence of C. papaya callus. The EC presented higher participation of alternative oxidase (AOX)enzymes, higher total cell respiration and presented a stronger accumulation of mitochondrial stress response proteins. Differential accumulation of auxin-responsive Gretchen Hagen 3 (GH3)family proteins in EC was related to a decrease in the content of free 2,4-dichlorophenoxyacetic acid (2,4-D). EC also showed higher endogenous H2O2 contents. H2O2 is a promising molecule for further investigation in differentiation protocols for C. papaya somatic embryos. HORMONES LABEL-FREE PROTEOMICS MITOCHONDRIAL PROTEOMICS SOMATIC EMBRYOS Vale, E. M. Santana, D. B. Reis, R. S. Sousa, K. R. De Souza Filho, G. A. De Oliveira, J. G. Silveira, V. https://drive.google.com/file/d/190bR6GiGSoJxqS01MbOaXcu3FJvWuM50/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 18719 18719 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-8476 2025-06-25 12:47:21 2025-06-25 REF1