01839nam a2200217Ia 4500003001000000005001700010040000900027245008600036490006300122520109600185650002001281650001501301650001901316650002101335650001401356700001901370700001801389700001701407856015601424008004101580MX-MdCICY20260521091111.0  cCICY10aEarly.flowering Scots pines through tissue culture for accelerating tree breeding0 vTheoretical and Applied Genetics, 93(5-6), p.840-848, 19963 aScots pine plantlets were produced via tissue culture using cotyledons excised from germinated embryos as ~xplants. The optimum tissue culture conditions were: ~4GD basal medium gelled with agar-Gelrite during shoot formation and with agar during rooting, inclusion of 5.0 btM benzylaminopurine (BAP)and 0.05 pM naphthaleneacetic acid (NAA)for 2 weeks for shoot induction, and repeated 2.7 pM NAA pulses of 1 week for rooting. Micropropagation success was genotype-dependent. Average multiplication rates varied among experiments from 3 to 15 shoots per embryo. The maximum shoot production from a single embryo was 35. Rooting was the most difficult phase in the propagation process. Most of the plantlets had a plagiotrophic and highly branched growth habit when growing in the greenhouse. Some individuals produced megasporangiate strobili at the age of 3 years and microsporangiate strobili with viable pollen at the age of 4 years. Early-flowering clones and the ability to onserve seedlings from which cotyledons have been cultured give new possibilities for accelerated tree breeding.14aPINUS SYHESTRIS14aSCOTS PINE14aTISSUE CULTURE14aEARLY MATURATION14aFLOWERING12aHaggman, H. M.12aAronen, T. S.12aStomp, A.-M.40uhttps://drive.google.com/file/d/1FBShW8tr00a2Q-ODH7uv2jOou-qsniVQ/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d