02517nam a2200253Ia 4500003001000000005001700010040000900027245012900036490004800165520163300213650001901846650001901865650002501884650001801909650002201927650002201949650002001971700002101991700001402012700001802026700002202044856015602066008004102222MX-MdCICY20260521091044.0  cCICY10aGenetic variation and evolution of Polaskia chichipe (Cactaceae)under domestication in the Tehuacán Valley, central Mexico0 vMolecular Ecology, 14(6), p.1603-1611, 20053 aPolaskia chichipe is a columnar cactus under artificial selection in central Mexico because of its edible fruits. Our study explored the effect of human manipulation on levels and distribution of genetic variation in wild, silviculturally managed and cultivated sympatric populations. Total genetic variation, estimated in nine populations with five microsatellite loci, was H T = 0.658 ± 0.026 SE, which was mainly distributed within populations ( H S = 0.646)with low differentiation among them ( F ST = 0.015). Fixation index ( F IS ) in all populations was positive, indicating a deficit of heterozygous individuals with respect to Hardy-Weinberg expectations. When populations were pooled by management type, the highest expected heterozygosity ( H E = 0.631 ± 0.031 SE)and the lowest fixation index ( F IS = 0.07)were observed in wild populations, followed by cultivated populations ( H E = 0.56 ± 0.03 SE, F IS = 0.14), whereas the lowest variation was found in silviculturally managed populations ( H E = 0.51 ± 0.05 SE, F IS = 0.17). Low differentiation among populations under different management types ( F ST 0.005, P < 0.04)was bserved. A pattern of migration among neighbouring populations, suggested from isolation by distance ( r 2 = 0.314, P < 0.01), may have contributed to omogenizing populations and counteracting the effects of artificial selection. P. chichipe, used and managed for at least 700 generations, shows morphological differentiation, changes in breeding system and seed germination patterns associated with human management, with only slight genetic differences detected by neutral markers.14aCOLUMNAR CACTI14aCROP EVOLUTION14aDINUCLEOTIDE REPEATS14aDOMESTICATION14aGENETIC RESOURCES14aGENETIC STRUCTURE14aMICROSATELLITES12aOtero-Arnaiz, A.12aCasas, A.12aHamrick, J.L.12aCruse-Sanders, J.40uhttps://drive.google.com/file/d/1Do6kFXEB9z-JR9GHOjwdoAC6s2Lg96kn/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d