01480nam a2200337Ia 4500003001000000005001700010040000900027245007000036490005600106520047100162650001900633650002700652650000900679650002000688650002400708650001300732650001500745650001200760700001900772700001700791700001700808700001800825700001700843700001500860700001300875700001300888700002500901700001900926856015600945008004101101MX-MdCICY20260521091034.0  cCICY10aImproved Protein Identification through the Use of Unstained Gels0 vJournal of Proteome Research, 4(3), p.992-997, 20053 aIn this work, a method for improved protein identification of low-abundance proteins using unstained gels, in combination with robotics and matrix-assisted laser desorption/ionization tandem mass spectrometry, has been developed and evaluated. Omitting the silverstaining process resulted in increased protein identification scores, an increase in the number of peptides observed in the MALDI mass spectrum, and improved quality of the tandem mass spectrometry data.14aUNSTAINED GELS14aPROTEIN IDENTIFICATION14aPAGE14aSILVER STAINING14aIMMUNOPRECIPITATION14aROBOTICS14aAUTOMATION14aMALDIMS12aLoiselle, D.R.12aThelin, W.R.12aParker, C.E.12aDicheva, N.D.12aKesner, B.A.12aMocanu, V.12aWang, F.12aWang, F.12aEsteban Warren, M.R.12aBorchers, C.H.40uhttps://drive.google.com/file/d/1Jp7fDqCjsST2gxGLGNyE4jp8i81XsUmj/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d