01499nam a2200241Ia 4500 MX-MdCICY 20260521091015.0 CICY B-6615 An easy approach for the purification of native TFIIH Journal of BioChemical and Biophysical Methods , 62(3), p.207-213, 2005 Transcriptional regulation depends on the appropriate set of positive and negative regulating signals in order to provide the correct gene expression. In vitro studies in eukaryotic gene expression over the last few years have provided a wealth of information about new factors involved in the regulation of genes. However, the dissection of this mechanism requires the addition of well-characterized general transcription factors; with the exception of TFIID and TFIIH, all others can easily be expressed in a recombinant form. Here we provide a simple methodology to obtain partially purified transcriptionally active TFIIH free from other general transcription factors and active in transcription. TRANSCRIPTION TFIIH GENERAL TRANSCRIPTION FACTORS PURIFICATION Castañoa, E. Flores, R. D. Rodriguez Zapata, L. C. https://drive.google.com/file/d/1qbmA2FmBizPNEowvhA8uFTFFIiiPkzN1/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 16894 16894 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-6615 2025-06-25 12:46:46 2025-06-25 REF1