02312nam a2200265Ia 4500 MX-MdCICY 20260521091013.0 CICY B-6554 Improved ajmalicine production and recovery from Catharanthus roseus suspensions with increased product removal rates BioChemical Engineering Journal, 21(2), p.253-258, 2004 The potential of strategies for increasing secondary metabolite accumulation from plant cell cultures may still be partially limited by unfavorable conditions for biosynthesis (i.e. feedback inhibition, unfavorable equilibrium state)or product metabolism. In this paper, the rate of product removal was altered and used as a tool for probing the impact of these limiting conditions on product accumulation, using the production of ajmalicine and serpentine from Catharanthus roseus suspensions as a model system. The method for altering the in situ product removal rate was simple; Amberlite® XAD-7HP resin was enclosed in either a porous or less porous material, and experiments confirmed that the product removal rate was higher with resin enclosed in the porous Miracloth versus the less porous nylon. When resin was added to suspensions, higher ajmalicine production and recovery were associated with Miracloth-enclosed resin which had higher product removal rates. This simple experiment illustrated that further improvements in production and recovery were possible, indicating that conditions unfavorable for biosynthesis and/or product metabolism existed in cultures with nylon-enclosed resin. These results also suggest the importance of optimizing the product removal rate for improving the overall production and recovery of secondary metabolites from plant cell cultures IN SITU PRODUCT REMOVAL PRODUCT ADSORPTION PLANT CELL CULTURES CATHARANTHUS ROSEUS AJMALICINE AMBERLITE®XAD-7HP RESIN Leng Wong, P. Royce, A.J. Lee-Parsons, C.W.T. https://drive.google.com/file/d/1qDmwyn6GpwKjqn1mAsZQ4MvYv55Jcm-1/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 16834 16834 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-6554 2025-06-25 12:46:45 2025-06-25 REF1