02095nam a2200253Ia 4500 MX-MdCICY 20260521091013.0 CICY B-6552 O2- from elicitor-induced oxidative burst is necessary for triggering phenylalanine ammonia-lyase activation and catharanthine synthesis in Catharanthus roseus cell cultures Enzyme and Microbial Technology, 36(2-3), p.280-284, 2005 Elicitor, derived from the cell walls of Aspergillus niger, induced rapid generation of reactive oxygen intermediates (ROI), including superoxide anion (O2-)and hydrogen peroxide (H2O2), sequentially followed by phenylalanine ammonia-lyase (PAL)activation and catharanthine biosynthesis in Catharanthus roseus suspension cells. The elicitor-induced PAL activation and catharanthine biosynthesis were blocked by NAD(P)H oxidase inhibitor, diphenylene iodonium (DPI). O2- generated by the reaction of xanthine/xanthine oxidase (X/XO)triggered PAL activation and catharanthine biosynthesis of C. roseus cells in the absence of elicitor and reversed the inhibitory effect of DPI on elicitor-induced PAL activation and catharanthine biosynthesis. External application of H2O2 and catalase had no effect on PAL activity and catharanthine contents of C. roseus cells. The results demonstrated a causal relationship between elicitor-induced oxidative burst and PAL activation in C. roseus suspension cells and suggested a sequence of signaling events from ROI production to PAL activation and catharanthine synthesis. Within this sequence, O2- rather than H2O2 appeared to trigger the subsequent reactions SUPEROXIDE ANIONS H2O2 ELICITOR CATHARANTHUS ROSEUS CELLS CATHARANTHINE PAL Xu, M. Dong, J. https://drive.google.com/file/d/19ao8nfc3SxD76rFDSNqfKRX5N4_v0HOr/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 16832 16832 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-6552 2025-06-25 12:46:45 2025-06-25 REF1