02137nam a2200253Ia 4500003001000000005001700010040000900027245010000036490003900136520131500175650001501490650001801505650002001523650002901543650001501572700001701587700001801604700001401622700001901636700001501655700001601670856015601686008004101842MX-MdCICY20260521091011.0  cCICY10aThe Pseudomonas phytotoxin coronatine mimics octadecanoid signalling molecules of higher plants0 vFEBS Letters, 345(1), p.9-13, 19943 aThe phytotoxic principle, coronatine, which is present in several pathovars of the plant pathogen, Pseudomonas syringae was shown to be highly active in completely different, jasmonate-selective bioassays. At nanomolar to micromolar concentrations, coronatine induced the accumulation of defense-related secondary metabolites in several plant cell cultures, induced transcript accumulation of the elicitor-responsive gene encoding the berberine bridge enzyme of Eschscholtzia californica, as well as the coiling response of Bryonia dioica tendrils. Biological activity critically depended upon the structure of coronatine, and slight modifications, such as methylation of the carboxyl moiety or reduction of the carbonyl group, rendered the molecules almost inactive. Coronafacic acid, obtained by hydrolysis of coronatine, was also nearly inactive. Coronatine did not elicit the accumulation of endogenous jasmonic acid in the systems analyzed. While coronafacic acid is similar in structure to jasmonic acid, we found coronatine to be a close structural analogue of the cyclic C18-precursor of jasmonic acid, 12-oxo-phytodienoic acid. The phytotoxic symptoms produced by coronatine can now be understood on the basis of the toxin's action as a mimic of the octadecanoid signalling molecules of higher plants.14aCORONATINE14aJASMONIC ACID14aMETHYLJASMONATE14a12-OXO-PHYTODIENOIC ACID14aCORONATINE12aWeiler, E.W.12aKutchan, T.M.12aGorba, T.12aBrodschelm, W.12aNiesel, U.12aBublitz, F.40uhttps://drive.google.com/file/d/1vKYRYko_2Tz0tGyJCUxONdmG7FT7aiIe/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d