02681nam a2200289Ia 4500003001000000005001700010040000900027090001100036245010900047490006700156520168100223650001101904650001501915650001901930650001001949700001701959700002001976700001701996700001802013700001302031700001702044856015602061942001402217008004102231999001702272952010202289MX-MdCICY20260521091010.0  cCICY  aB-644110aSynteny in Toxigenic Fusarium Species: The Fumonisin Gene Cluster and the Mating Type Region as Examples0 vEuropean Journal of Plant Pathology, 110(5-6), p.533-544, 20043 aA comparative genomic approach was used to study the mating type locus and the gene cluster involved in toxin production (fumonisin)in Fusarium proliferatum, a pathogen with a wide host range and a complex toxin profile. A BAC library, generated from F. proliferatum isolate ITEM 2287, was used to identify chromosomal regions flanking the mating type locus and the gene cluster involved in the biosynthesis of fumonisin. These regions were sequenced and compared with corresponding sequences in other ascomycetes. The results demonstrated that the level of synteny between ascomycetes can vary greatly for different genomic regions and that the level of similarity of genes within a region can also fluctuate strongly. Synteny was found in the regions flanking the mating type idiomorph among ascomycetes that supposedly diverged 280 million years ago. The fumonisin gene clusters of F. proliferatum and F. verticillioides were completely syntenic but absent in F. graminearum. The regions flanking the fumonisin gene clusters were highly dissimilar between F. proliferatum and F. verticillioides, whereas they formed a continuous region in F. graminearum. This indicates that the fumonisin gene cluster has been inserted at different genome locations in both species. Surprisingly low similarity was found between the corresponding genes within the fumonisin cluster of F. proliferatum and F. verticillioides, compared to other genomic sequences indicative for two independent acquisition events from distinct genetic sources. The results demonstrate the power of comparative genomics for gene annotation and for studies on the evolution of genes, gene-clusters and species.14aGENOME14aGIBBERELLA14aPLANT PATHOGEN14aTOXIN12aWaalwijk, C.12avan der Lee, T.12ade Vries, I.12aHesselink, T.12aArts, J.12aKema, G.H.J.40uhttps://drive.google.com/file/d/1u4AhcXCtzX2fvtBg3GQ8G5KMQR9MsGuY/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx  2LoccREF1250602s9999    xx |||||s2   |||| ||und|d  c16724d16724  00102Loc40708F1aCICYbCICYcREd2025-06-25l0oB-6441r2025-06-25 12:46:43w2025-06-25yREF1