01942nam a2200253Ia 4500003001000000005001700010040000900027245015100036490004900187520107500236650002301311650000901334650002101343650001601364650002001380700001501400700001701415700001601432700001401448700001401462700001501476856015601491008004101647MX-MdCICY20260521091008.0  cCICY10aSubstance P-induced activation of p42/44 mitogen-activated protein kinase associated with cell proliferation in human tracheal smooth muscle cells0 vCellular Signalling, 14(11), p.913-923, 20023 aSubstance P (SP)released from sensory nerve endings in the airways induces several responses including cell proliferation. However, the mechanisms were not completely understood in tracheal smooth muscle cells (TSMCs). We therefore investigated the effect of SP on cell proliferation and activation of p42/p44 mitogen-activated protein kinase (MAPK)in these cells. SP stimulated [3H]thymidine incorporation and p42/p44 MAPK phosphorylation in a time- and concentration-dependent manner in TSMCs. Both DNA synthesis and phosphorylation of MAPK in response to SP were attenuated by pretreatment with pertussis toxin, genistein, D609, U73122, staurosporine, removal of Ca2+ by BAPTA/AM plus EGTA, PD98059, and SB202190. Furthermore, overexpression of dominant negative mutants, H-Ras-15A and Raf-N4, significantly suppressed p42/p44 MAPK activation induced by SP and PDGF-BB. These results conclude that the mitogenic effect of SP was mediated through the activation of Ras/Raf/MEK/MAPK pathway, which was modulated by PC-PLC, PI-PLC, Ca2+, and PKC in cultured human TSMCs.14aCELL PROLIFERATION14aMAPK14aPROTEIN KINASE C14aSUBSTANCE P14aTYROSINE KINASE12aYang, C-M.12aHsiao, L.-D.12aChien, C-S.12aLin, C-C.12aLuo, S-F.12aWang, C-C.40uhttps://drive.google.com/file/d/105JNXk3lOpzeL-OPfHSaKz5owQs5v_gJ/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d