02477nam a2200337Ia 4500003001000000005001700010040000900027090001100036245011500047490003900162520139500201650001001596650001401606650002701620650001001647650001101657650001601668650001201684650001401696700001801710700001501728700001501743700001801758700001501776700001801791856015601809942001401965008004101979999001702020952010202037MX-MdCICY20260521090946.0  cCICY  aB-568110amiR775 integrates light, sucrose and auxin associated pathways to regulate root growth in Arabidopsis thaliana0 vPlant Science, 313, p.111073, 20213 aMicroRNAs (miRNAs), a class of single-stranded non-coding RNA of 20-24 nucleotides, regulate gene expression by target gene transcript cleavage or translation inhibition. The phytohormone auxin is a crucial regulator of almost every process involved in plant growth and development. Several studies have demonstrated the involvement of miRNA(s)in the regulation of the auxin signaling pathway and plant development. However, very few studies have identified the auxin-mediated regulation of miRNA(s). In this study, we reveal the detailed mechanism of auxin-mediated regulation of the cell wall-related miR775- Galactosyl transferase (GalT)module, which plays an important role in root growth in Arabidopsis thaliana. We also showed two interdependent mechanisms by which miR775 regulates root growth: miR775-GalT and light-mediated sucrose-dependent pathways. Treatment of GUS reporter lines with Indole Acetic Acid (IAA), sucrose, and light apparently enhanced the abundance of miR775 in root tissue. miR775 overexpressing (miR775OX)lines showed changes in root architecture, including increased primary root growth and root hair, by targeting GalT. miR775OX lines also showed tolerance toward low Pi. These results provide new insights into the auxin regulation of cell wall-related miR775 and suggest its significant role in plant root growth and development by modifying the cell wall.14aAUXIN14aCELL WALL14aGALACTOSYL TRANSFERASE14aLIGHT14aMIR77514aROOT GROWTH14aSUCROSE14aPHOSPHATE12aGaddam, S. R.12aBhatia, C.12aSharma, A.12aBadola, P. K.12aSaxena, G.12aTrivedi, P. K40uhttps://drive.google.com/file/d/1_vZ-1Yz6DFocyo8kcXNAmT_WhMsh2YRJ/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx  2LoccREF1250602s9999    xx |||||s2   |||| ||und|d  c15966d15966  00102Loc40708F1aCICYbCICYcREd2025-06-25l0oB-5681r2025-06-25 12:25:03w2025-06-25yREF1