02363nam a2200325Ia 4500 MX-MdCICY 20260521090946.0 CICY B-5681 miR775 integrates light, sucrose and auxin associated pathways to regulate root growth in Arabidopsis thaliana Plant Science, 313, p.111073, 2021 MicroRNAs (miRNAs), a class of single-stranded non-coding RNA of 20-24 nucleotides, regulate gene expression by target gene transcript cleavage or translation inhibition. The phytohormone auxin is a crucial regulator of almost every process involved in plant growth and development. Several studies have demonstrated the involvement of miRNA(s)in the regulation of the auxin signaling pathway and plant development. However, very few studies have identified the auxin-mediated regulation of miRNA(s). In this study, we reveal the detailed mechanism of auxin-mediated regulation of the cell wall-related miR775- Galactosyl transferase (GalT)module, which plays an important role in root growth in Arabidopsis thaliana. We also showed two interdependent mechanisms by which miR775 regulates root growth: miR775-GalT and light-mediated sucrose-dependent pathways. Treatment of GUS reporter lines with Indole Acetic Acid (IAA), sucrose, and light apparently enhanced the abundance of miR775 in root tissue. miR775 overexpressing (miR775OX)lines showed changes in root architecture, including increased primary root growth and root hair, by targeting GalT. miR775OX lines also showed tolerance toward low Pi. These results provide new insights into the auxin regulation of cell wall-related miR775 and suggest its significant role in plant root growth and development by modifying the cell wall. AUXIN CELL WALL GALACTOSYL TRANSFERASE LIGHT MIR775 ROOT GROWTH SUCROSE PHOSPHATE Gaddam, S. R. Bhatia, C. Sharma, A. Badola, P. K. Saxena, G. Trivedi, P. K https://drive.google.com/file/d/1_vZ-1Yz6DFocyo8kcXNAmT_WhMsh2YRJ/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 15966 15966 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-5681 2025-06-25 12:25:03 2025-06-25 REF1