Jacobs, D van Rijssen, M.S van der Heijsen, R Verpoorte, R. text xx 9999 monographic und

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Sample preparation is still the most critical step in two-dimensional gel electrophoresis (2-DE), and needs to be optimized for each type of sample. To analyze the proteome of the medicinal plant Catharanthus roseus, we developed and evaluated a sequential solubilization procedure for the solubilization of proteins after precipitation in trichloroacetic acid and acetone. The procedure includes solubilization with a conventional urea buffer followed by a stronger solubilizing buffer containing thiourea. The sequential solubilization of the precipitated proteins results in very different spot patterns following 2-DE. The number of protein spots which could be detected in both samples of the sequential solubilization was only about 10 https://drive.google.com/file/d/1IDhK4yTinMNx3FiDKc29t9lFkwSL20Ei/view?usp=drivesdk https://drive.google.com/file/d/1IDhK4yTinMNx3FiDKc29t9lFkwSL20Ei/view?usp=drivesdk 250602 20260521090923.0