01687nam a2200229Ia 4500 MX-MdCICY 20260521090923.0 CICY B-4959 Geraniol 10-hydroxylase, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis FEBS Letters, 508, p.215-220, 2001 Geraniol 10-hydroxylase (G10H)is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid alkaloids found in a diverse range of plant species. Catharanthus roseus (Madagascar periwinkle)contains monoterpenoid indole alkaloids, several of which are pharmaceutically important. Vinblastine and vincristine, for example, find widespread use as anti-cancer drugs. G10H is thought to play a key regulatory role in terpenoid indole alkaloid biosynthesis. We purified G10H from C. roseus cells. Using degenerate PCR primers based on amino acid sequence information we cloned the corresponding cDNA. The encoded CYP76B6 protein has G10H activity when expressed in C. roseus and yeast cells. The stress hormone methyljasmonate strongly induced G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in a C. roseus cell culture. Collu, G Unver, N Peltenburg-Looman, A.M.G. Heijen van der, R Verpoorte, R. Memelink, J. https://drive.google.com/file/d/17belGS5t6gL_QiiRADTIoR_-u3SmwSy9/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 15257 15257 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-4959 2025-06-25 12:24:50 2025-06-25 REF1