Wang, X. Wang, Z. Wang, Q. Liang, H. Liu, D. text xx 9999 monographic und

electronic

To explore the effect of epigenetic modification on the differentiation of goat adipose-derived stem cells in vitro, we used two common epigenetic modification inhibitors, trichostatin A and vorinostat, to treat cashmere goat adipose-derived stem cells and induce adipocyte differentiation. The results showed that trichostatin A and vorinostat changed the relative amounts of H3K9 acetylation and dimethylation in the upstream sequence of PPARG, increased peroxisome proliferator-activated receptor gamma (PPARG)transcription before differentiation and then promoted adipocyte differentiation, and regulated the expression of adipocyte-specific genes. We conclude that adipocyte differentiation is regulated dynamically by different histone modifications. The areas of acetylation and demethylation changed by trichostatin A and vorinostat are the basis for further research on the mechanism of PPARG promoter to regulate adipocytes differentiation and provide research theroies for using adipose-derived stem cells as donor to produce transgenic animals to improve meat quality improvement. ACETYLATION ADIPOGENIC DIFFERENTIATION ADIPOSE-DERIVED STEM CELLS DIMETHYLATION GOAT https://drive.google.com/file/d/1S-lPYqOR5vk2dkl2Ba4VZ1RR3w1u5pH2/view?usp=drivesdk https://drive.google.com/file/d/1S-lPYqOR5vk2dkl2Ba4VZ1RR3w1u5pH2/view?usp=drivesdk 250602 20260521090843.0