Higher power density is a key technological goal for the deployment of polymer electrolyte membrane fuel cells (PEMFCs) in vehicles. Water production is proportional to current density and at high current densities water flooding in catalyst layers can result in significant mass transport limitations. A novel perfluoropolymers material, Teflon™ AF, is introduced as a hydrophobic agent to fabricate microporous layers (MPLs). The MPL ink with varying proportions of Teflon™ AF and Vulcan XC-72 carbon black is prepared and used to coat MPLs into the gas diffusion layers. X-ray computed tomography and mercury intrusion porosimetry are used to characterize the physical properties of the penetrated GDM. The performance of cells with the homemade MPLs are compared with commercial GDM cells under dry and wet operating conditions using varying backpressures. The results at high relative humidity and high backpressure show that the cells with homemade MPLs have less flooding than that with commercial GDM. Oxygen transport resistance measurements using the limiting current method show that the penetration of the MPL into the substrate has an undesirable effect of increasing oxygen transport resistance. The results are of benefit to understanding the water management and gas transport mechanism of MPL for fuel cells operating at both dry and wet conditions. © 2024 Elsevier B.V.