Somatic Embryogenesis in Tissues From Mesophyll and Cell Suspension Protoplasts of Medicago coerulea and M. glutinosa
Material type:
TextSeries: ; Z. Pjlanzenphysiol., 106, p.105-110, 1982Contained works: - Arcioni, S
- Davey, M. R
- Dos Santos, A. V. P
- Cocking, E. C
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Mesophyll and cell suspension protoplasts of Medicago coerulea and M glutinosa divided to form callus. The conditions of plant growth, plant age, protoplast isolation, and protoplast cul-ture were critical for sustained division of mesophyll protoplasts. Those for division of cell suspension protoplasts were less stringent. 2,4-D in combination with a cytokinin induced embryogenesis in both Medicago species. In M. coerulea, BAP was effective as the cytokinin, while M. glutinosa had a requirement for zeatin. The potential of protoplast systems for transfer of characteristics from M. coerulea to M. glutinosa and M. sativa is discussed.
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