Increased accumulation of anthocyanins in transgenic potato tubers by overexpressing the 3GT gene
Material type:
TextSeries: ; Plant Biotechnol Rep, 6, p.69-75, 2012Contained works: - Wei, Q
- Wang, Q-Y
- Feng, Z-H
- Wang, B
- Zhang, Y-F
- Yang, Q
| Cover image | Item type | Current library | Home library | Collection | Shelving location | Call number | Materials specified | Vol info | URL | Copy number | Status | Notes | Date due | Barcode | Item holds | Item hold queue priority | Course reserves | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| REF1 | CICY | F1 | B-14465 (Browse shelf(Opens below)) | Available |
In order to explore a biotechnological method for improving potato tuber color and creating plants with increased anthocyanin contents, a potato UDP-glucose: flavonoid-3-O-glucosyltransferase (3GT)gene was inserted behind the GBSSI prom oter of pBin19, and this construct was introduced into Solanum tuberosum L. cultivar De´sire´e plants by Agrobacterium-mediated transformation. Six independent transgenic lines overexpressing the 3GT gene were identified by PCR and Southern blot analysis from 18 kanamycin-resistant plants. Due to the expression of 3GT gene, the tuber color and the anthocyanin content were enhanced noticeably in the transgenic plants compared to the wild-type control plants. This result suggests that the 3GT gene can potentially be used to improve potato color and enhance levels of antioxidants in the diet
There are no comments on this title.