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Efficient production of human acidic fibroblast growth factor in pea (Pisum sativum L.)plants by agroinfection of germinated seeds

Material type: TextSeries: ; BMC Biotechnology, 11(1), p.45, 2011Contained works:
  • Fan, Y
  • Li, W
  • Wang, J
  • Liu, J
  • Yang, M
  • Xu, D
  • Zhu, X
  • Zhu, X
Online resources: Abstract: For efficient and large scale production of recombinant proteins in plants transient expression by agroinfection has a number of advantages over stable transformation. Simple manipulation_Crapid analysis and high expression efficiency are possible. In pea, Pisum sativum, a Virus Induced Gene Silencing System using the pea early browning virus has been converted into an efficient agroinfection system by converting the two RNA genomes of the virus into binary expression vectors for Agrobacterium transformation.
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For efficient and large scale production of recombinant proteins in plants transient expression by agroinfection has a number of advantages over stable transformation. Simple manipulation_Crapid analysis and high expression efficiency are possible. In pea, Pisum sativum, a Virus Induced Gene Silencing System using the pea early browning virus has been converted into an efficient agroinfection system by converting the two RNA genomes of the virus into binary expression vectors for Agrobacterium transformation.

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