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Selective inhibition of cytosine-DNA methylases by polyamines

Material type: TextSeries: ; FEBS Letters, 357(2), p.192-196, 1995Contained works:
  • Ruiz-Herrera, J
  • Ruiz-Medrano, R
  • Dominguez, A
Subject(s): Online resources: Abstract: We have advanced the hypothesis that polyamines affect DNA methylation and thus promote the expression of developmentally controlled genes. We demonstrate that the activity of cytosine-DNA methyltransferases HpaII, HhaI, HaeIII and SssI is inhibited by physiological concentrations of polyamines. On the other hand, activity of the adenine-DNA methyltransferase EcoRI, and restriction enzymes HpaII, HhaI, HaeIII and EcoRI, is insensitive to polyamine concentrations up to 40 mM. Our results indicate that the effect of polyamines on cytosine- DNA methyltranferases is rather selective and suggest a possible mode of action in vivo.
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We have advanced the hypothesis that polyamines affect DNA methylation and thus promote the expression of developmentally controlled genes. We demonstrate that the activity of cytosine-DNA methyltransferases HpaII, HhaI, HaeIII and SssI is inhibited by physiological concentrations of polyamines. On the other hand, activity of the adenine-DNA methyltransferase EcoRI, and restriction enzymes HpaII, HhaI, HaeIII and EcoRI, is insensitive to polyamine concentrations up to 40 mM. Our results indicate that the effect of polyamines on cytosine- DNA methyltranferases is rather selective and suggest a possible mode of action in vivo.

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