IHneclreenaese Vdi goeilo alaccs uemt aull.ation in seeds Increasing seed oil content in oil-seed rape ( Brassica napus L.)by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter
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TextSeries: ; Plant Biotechnology Journal, 5(3), p.431-441, 2007Contained works: - Vigeolas, H
- Waldeck, P
- Zank, T
- Geigenberger, P
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Previous attempts to manipulate oil synthesis in plants have mainly concentrated on the genes involved in the biosynthesis and use of fatty acids, neglecting the possible role of glycerol-3-phosphate supply on the rate of triacylglycerol synthesis. In this study, a yeast gene coding for cytosolic glycerol-3-phosphate dehydrogenase ( gpd 1)was expressed in transgenic oil-seed rape under the control of the seed-specific napin promoter. It was found that a twofold increase in glycerol-3-phosphate dehydrogenase activity led to a three- to fourfold increase in the level of glycerol-3-phosphate in developing seeds, resulting in a 40
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